Construct: shRNA TRCN0000092558

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_489354.1-688s1c1
DNA Barcode:
GCTCGACATGACATTCAAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC434815 (434815)
Gene Description:
hypothetical gene supported by NM_146237
Transcript:
RefSeq XM_489354.1 (NON-CURRENT)
Match location:
Position 688 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000092558)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCGACATGACATTCAAATT
Hairpin Sequence:
5'-CCGG-GCTCGACATGACATTCAAATT-CTCGAG-AATTTGAATGTCATGTCGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCGACATGACATTCAAATTCTCGAGAATTTGAATGTCATGTCGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCGACATGACATTCAAATTCTCGAGAATTTGAATGTCATGTCGAGC-3'

Other clones with same target sequence:

(none)