Construct: shRNA TRCN0000092583

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_110701.2-948s1c1
DNA Barcode:
CCCACCAAATTCAACTGTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmprss11a (194597)
Gene Description:
transmembrane protease, serine 11a
Transcript:
RefSeq XM_110701.2 (NON-CURRENT)
Match location:
Position 948 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000092583)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCACCAAATTCAACTGTCTA
Hairpin Sequence:
5'-CCGG-CCCACCAAATTCAACTGTCTA-CTCGAG-TAGACAGTTGAATTTGGTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCACCAAATTCAACTGTCTACTCGAGTAGACAGTTGAATTTGGTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCACCAAATTCAACTGTCTACTCGAGTAGACAGTTGAATTTGGTGGG-3'

Other clones with same target sequence:

(none)