Construct: shRNA TRCN0000092589

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_355199.1-2004s1c1
DNA Barcode:
GCGATCTAACTCCATACAGCA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hecw2 (329152)
Gene Description:
HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2
Transcript:
RefSeq XM_355199.1 (NON-CURRENT)
Match location:
Position 2004 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000092589)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGATCTAACTCCATACAGCA
Hairpin Sequence:
5'-CCGG-GCGATCTAACTCCATACAGCA-CTCGAG-TGCTGTATGGAGTTAGATCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGATCTAACTCCATACAGCACTCGAGTGCTGTATGGAGTTAGATCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGATCTAACTCCATACAGCACTCGAGTGCTGTATGGAGTTAGATCGC-3'

Other clones with same target sequence:

(none)