Construct: shRNA TRCN0000092671

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178624.2-428s1c1
DNA Barcode:
CCTTAGCAGATTCTGTTCCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fbxl2 (72179)
Gene Description:
F-box and leucine-rich repeat protein 2
Transcript:
RefSeq NM_178624.2 (NON-CURRENT)
Match location:
Position 428 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000092671)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTAGCAGATTCTGTTCCAA
Hairpin Sequence:
5'-CCGG-CCTTAGCAGATTCTGTTCCAA-CTCGAG-TTGGAACAGAATCTGCTAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTAGCAGATTCTGTTCCAACTCGAGTTGGAACAGAATCTGCTAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTAGCAGATTCTGTTCCAACTCGAGTTGGAACAGAATCTGCTAAGG-3'

Other clones with same target sequence:

(none)