Construct: shRNA TRCN0000092681

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_112098.1-169s1c1
DNA Barcode:
CAGCTACCAAAGTCCAAACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Serpina16 (194604)
Gene Description:
serine (or cysteine) peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 16
Transcript:
RefSeq XM_112098.1 (NON-CURRENT)
Match location:
Position 169 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000092681)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCTACCAAAGTCCAAACTA
Hairpin Sequence:
5'-CCGG-CAGCTACCAAAGTCCAAACTA-CTCGAG-TAGTTTGGACTTTGGTAGCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCTACCAAAGTCCAAACTACTCGAGTAGTTTGGACTTTGGTAGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCTACCAAAGTCCAAACTACTCGAGTAGTTTGGACTTTGGTAGCTG-3'

Other clones with same target sequence:

(none)