Construct: shRNA TRCN0000092693
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_485920.1-1031s1c1
- DNA Barcode:
- GCACAGCACAAATTAGTTATA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Hmgb1-ps7 (434174)
- Gene Description:
- high-mobility group high mobility group box 1, pseudogene 7
- Transcript:
- RefSeq XM_485920.1 (NON-CURRENT)
- Match location:
- Position 1031 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000092693)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCACAGCACAAATTAGTTATA
- Hairpin Sequence:
-
5'-CCGG-GCACAGCACAAATTAGTTATA-CTCGAG-TATAACTAATTTGTGCTGTGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCACAGCACAAATTAGTTATACTCGAGTATAACTAATTTGTGCTGTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCACAGCACAAATTAGTTATACTCGAGTATAACTAATTTGTGCTGTGC-3'
Other clones with same target sequence:
(none)
