Construct: shRNA TRCN0000092696

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_485920.1-727s1c1
DNA Barcode:
GAAGATGAAGAAGAAGATGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hmgb1-ps7 (434174)
Gene Description:
high-mobility group high mobility group box 1, pseudogene 7
Transcript:
RefSeq XM_485920.1 (NON-CURRENT)
Match location:
Position 727 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000092696)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGATGAAGAAGAAGATGAT
Hairpin Sequence:
5'-CCGG-GAAGATGAAGAAGAAGATGAT-CTCGAG-ATCATCTTCTTCTTCATCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGATGAAGAAGAAGATGATCTCGAGATCATCTTCTTCTTCATCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGATGAAGAAGAAGATGATCTCGAGATCATCTTCTTCTTCATCTTC-3'

Other clones with same target sequence:

(none)