Construct: shRNA TRCN0000092711

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_357228.2-145s1c1
DNA Barcode:
TGGGAGACCATGTCTGCTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC383761 (383761)
Gene Description:
similar to Hmgb1 protein
Transcript:
RefSeq XM_357228.2 (NON-CURRENT)
Match location:
Position 145 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000092711)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGGAGACCATGTCTGCTAAA
Hairpin Sequence:
5'-CCGG-TGGGAGACCATGTCTGCTAAA-CTCGAG-TTTAGCAGACATGGTCTCCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGGAGACCATGTCTGCTAAACTCGAGTTTAGCAGACATGGTCTCCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGGAGACCATGTCTGCTAAACTCGAGTTTAGCAGACATGGTCTCCCA-3'

Other clones with same target sequence:

(none)