Construct: shRNA TRCN0000092729

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175655.1-143s1c1
DNA Barcode:
CCGGTCTCATCTACGAGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hist1h4f (319157)
Gene Description:
histone cluster 1, H4f
Transcript:
RefSeq NM_175655.1 (NON-CURRENT)
Match location:
Position 143 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000092729)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGTCTCATCTACGAGGAAA
Hairpin Sequence:
5'-CCGG-CCGGTCTCATCTACGAGGAAA-CTCGAG-TTTCCTCGTAGATGAGACCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGTCTCATCTACGAGGAAACTCGAGTTTCCTCGTAGATGAGACCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGTCTCATCTACGAGGAAACTCGAGTTTCCTCGTAGATGAGACCGG-3'

Other clones with same target sequence:

(none)