Construct: shRNA TRCN0000092733

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_356549.1-183s1c1
DNA Barcode:
GCTGATTCGCAAGTTGCCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm12260 (382523)
Gene Description:
predicted gene 12260
Transcript:
RefSeq XM_356549.1 (NON-CURRENT)
Match location:
Position 183 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000092733)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGATTCGCAAGTTGCCATT
Hairpin Sequence:
5'-CCGG-GCTGATTCGCAAGTTGCCATT-CTCGAG-AATGGCAACTTGCGAATCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGATTCGCAAGTTGCCATTCTCGAGAATGGCAACTTGCGAATCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGATTCGCAAGTTGCCATTCTCGAGAATGGCAACTTGCGAATCAGC-3'

Other clones with same target sequence:

(none)