Construct: shRNA TRCN0000092759

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013676.1-2211s1c1
DNA Barcode:
CCAGAGTAGAACTGCATTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Supt5 (20924)
Gene Description:
suppressor of Ty 5, DSIF elongation factor subunit
Transcript:
RefSeq NM_013676.1 (CURRENT)
Match location:
Position 2211 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000092759)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAGTAGAACTGCATTCTA
Hairpin Sequence:
5'-CCGG-CCAGAGTAGAACTGCATTCTA-CTCGAG-TAGAATGCAGTTCTACTCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAGTAGAACTGCATTCTACTCGAGTAGAATGCAGTTCTACTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAGTAGAACTGCATTCTACTCGAGTAGAATGCAGTTCTACTCTGG-3'

Other clones with same target sequence:

(none)