Construct: shRNA TRCN0000092776

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175663.1-45s1c1
DNA Barcode:
CTTCAAGAAAGCGGTCACCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hist1h2ba (319177)
Gene Description:
histone cluster 1, H2ba
Transcript:
RefSeq NM_175663.1 (NON-CURRENT)
Match location:
Position 45 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000092776)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCAAGAAAGCGGTCACCAA
Hairpin Sequence:
5'-CCGG-CTTCAAGAAAGCGGTCACCAA-CTCGAG-TTGGTGACCGCTTTCTTGAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCAAGAAAGCGGTCACCAACTCGAGTTGGTGACCGCTTTCTTGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCAAGAAAGCGGTCACCAACTCGAGTTGGTGACCGCTTTCTTGAAG-3'

Other clones with same target sequence:

(none)