Construct: shRNA TRCN0000092797

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013673.1-1433s1c1
DNA Barcode:
CCTGAGAGAACTGATACAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sp100 (20684)
Gene Description:
nuclear antigen Sp100
Transcript:
RefSeq NM_013673.1 (NON-CURRENT)
Match location:
Position 1433 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000092797)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAGAGAACTGATACAGAA
Hairpin Sequence:
5'-CCGG-CCTGAGAGAACTGATACAGAA-CTCGAG-TTCTGTATCAGTTCTCTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAGAGAACTGATACAGAACTCGAGTTCTGTATCAGTTCTCTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAGAGAACTGATACAGAACTCGAGTTCTGTATCAGTTCTCTCAGG-3'

Other clones with same target sequence:

(none)