Construct: shRNA TRCN0000092798

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026189.3-2296s1c1
DNA Barcode:
GTCCACTTTGTGAACACTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eepd1 (67484)
Gene Description:
endonuclease/exonuclease/phosphatase family domain containing 1
Transcript:
RefSeq NM_026189.3 (CURRENT)
Match location:
Position 2296 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000092798)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCCACTTTGTGAACACTTTA
Hairpin Sequence:
5'-CCGG-GTCCACTTTGTGAACACTTTA-CTCGAG-TAAAGTGTTCACAAAGTGGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCCACTTTGTGAACACTTTACTCGAGTAAAGTGTTCACAAAGTGGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCCACTTTGTGAACACTTTACTCGAGTAAAGTGTTCACAAAGTGGAC-3'

Other clones with same target sequence:

(none)