Construct: shRNA TRCN0000092855

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178216.1-363s1c1
DNA Barcode:
GCAGGACTTCAAGACGGACCT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hist2h3c1 (15077)
Gene Description:
histone cluster 2, H3c1
Transcript:
RefSeq NM_178216.1 (NON-CURRENT)
Match location:
Position 363 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000092855)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGACTTCAAGACGGACCT
Hairpin Sequence:
5'-CCGG-GCAGGACTTCAAGACGGACCT-CTCGAG-AGGTCCGTCTTGAAGTCCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGACTTCAAGACGGACCTCTCGAGAGGTCCGTCTTGAAGTCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGACTTCAAGACGGACCTCTCGAGAGGTCCGTCTTGAAGTCCTGC-3'

Other clones with same target sequence:

(none)