Construct: shRNA TRCN0000092891

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015781.2-1017s1c1
DNA Barcode:
AGCCTTTATTTGATAAGCGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nap1l1 (53605)
Gene Description:
nucleosome assembly protein 1-like 1
Transcript:
RefSeq NM_015781.2 (NON-CURRENT)
Match location:
Position 1017 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000092891)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCCTTTATTTGATAAGCGAT
Hairpin Sequence:
5'-CCGG-AGCCTTTATTTGATAAGCGAT-CTCGAG-ATCGCTTATCAAATAAAGGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCCTTTATTTGATAAGCGATCTCGAGATCGCTTATCAAATAAAGGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCCTTTATTTGATAAGCGATCTCGAGATCGCTTATCAAATAAAGGCT-3'

Other clones with same target sequence:

(none)