Construct: shRNA TRCN0000092918

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008211.2-2706s1c1
DNA Barcode:
CCACAGTGTAACAGTTTCCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
H3f3b (15081)
Gene Description:
H3 histone, family 3B
Transcript:
RefSeq NM_008211.2 (NON-CURRENT)
Match location:
Position 2706 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000092918)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAGTGTAACAGTTTCCTT
Hairpin Sequence:
5'-CCGG-CCACAGTGTAACAGTTTCCTT-CTCGAG-AAGGAAACTGTTACACTGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAGTGTAACAGTTTCCTTCTCGAGAAGGAAACTGTTACACTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAGTGTAACAGTTTCCTTCTCGAGAAGGAAACTGTTACACTGTGG-3'

Other clones with same target sequence:

  1. TRCN0000309364