Construct: shRNA TRCN0000092929

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_485484.1-751s1c1
DNA Barcode:
GAGGAAGATGAAGAGGAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC433788 (433788)
Gene Description:
similar to high mobility group protein B2
Transcript:
RefSeq XM_485484.1 (NON-CURRENT)
Match location:
Position 751 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000092929)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGAAGATGAAGAGGAAGAA
Hairpin Sequence:
5'-CCGG-GAGGAAGATGAAGAGGAAGAA-CTCGAG-TTCTTCCTCTTCATCTTCCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGAAGATGAAGAGGAAGAACTCGAGTTCTTCCTCTTCATCTTCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGAAGATGAAGAGGAAGAACTCGAGTTCTTCCTCTTCATCTTCCTC-3'

Other clones with same target sequence:

(none)