Construct: shRNA TRCN0000092956

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031842.1-1041s1c1
DNA Barcode:
GCGGATGAAGTTCTCAGAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smarcd1 (83797)
Gene Description:
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1
Transcript:
RefSeq NM_031842.1 (NON-CURRENT)
Match location:
Position 1041 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000092956)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGATGAAGTTCTCAGAGAT
Hairpin Sequence:
5'-CCGG-GCGGATGAAGTTCTCAGAGAT-CTCGAG-ATCTCTGAGAACTTCATCCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGATGAAGTTCTCAGAGATCTCGAGATCTCTGAGAACTTCATCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGATGAAGTTCTCAGAGATCTCGAGATCTCTGAGAACTTCATCCGC-3'

Other clones with same target sequence:

(none)