Construct: shRNA TRCN0000093052

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030082.1-138s1c1
DNA Barcode:
GAGCTACTCCATCTACGTGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hist3h2ba (78303)
Gene Description:
histone cluster 3, H2ba
Transcript:
RefSeq NM_030082.1 (NON-CURRENT)
Match location:
Position 138 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093052)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCTACTCCATCTACGTGTA
Hairpin Sequence:
5'-CCGG-GAGCTACTCCATCTACGTGTA-CTCGAG-TACACGTAGATGGAGTAGCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCTACTCCATCTACGTGTACTCGAGTACACGTAGATGGAGTAGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCTACTCCATCTACGTGTACTCGAGTACACGTAGATGGAGTAGCTC-3'

Other clones with same target sequence:

(none)