Construct: shRNA TRCN0000093058

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175665.1-192s1c1
DNA Barcode:
CTCGTTCGTGAACGACATCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hist1h2bk (319184)
Gene Description:
histone cluster 1, H2bk
Transcript:
RefSeq NM_175665.1 (NON-CURRENT)
Match location:
Position 192 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093058)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCGTTCGTGAACGACATCTT
Hairpin Sequence:
5'-CCGG-CTCGTTCGTGAACGACATCTT-CTCGAG-AAGATGTCGTTCACGAACGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCGTTCGTGAACGACATCTTCTCGAGAAGATGTCGTTCACGAACGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCGTTCGTGAACGACATCTTCTCGAGAAGATGTCGTTCACGAACGAG-3'

Other clones with same target sequence:

(none)