Construct: shRNA TRCN0000093073

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_485250.2-798s1c1
DNA Barcode:
GCCTCTGAAGAAGAGAAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC433602 (433602)
Gene Description:
similar to Nonhistone chromosomal protein HMG-14 (High-mobility group nucleosome binding domain 1)
Transcript:
RefSeq XM_485250.2 (NON-CURRENT)
Match location:
Position 798 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093073)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCTGAAGAAGAGAAAGAA
Hairpin Sequence:
5'-CCGG-GCCTCTGAAGAAGAGAAAGAA-CTCGAG-TTCTTTCTCTTCTTCAGAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCTGAAGAAGAGAAAGAACTCGAGTTCTTTCTCTTCTTCAGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCTGAAGAAGAGAAAGAACTCGAGTTCTTTCTCTTCTTCAGAGGC-3'

Other clones with same target sequence:

(none)