Construct: shRNA TRCN0000093162

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_134164.2-524s1c1
DNA Barcode:
CCTTAGTATAGAAGACACCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Syt12 (171180)
Gene Description:
synaptotagmin XII
Transcript:
RefSeq NM_134164.2 (NON-CURRENT)
Match location:
Position 524 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093162)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTAGTATAGAAGACACCTT
Hairpin Sequence:
5'-CCGG-CCTTAGTATAGAAGACACCTT-CTCGAG-AAGGTGTCTTCTATACTAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTAGTATAGAAGACACCTTCTCGAGAAGGTGTCTTCTATACTAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTAGTATAGAAGACACCTTCTCGAGAAGGTGTCTTCTATACTAAGG-3'

Other clones with same target sequence:

(none)