Construct: shRNA TRCN0000093182

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_197943.1-2525s1c1
DNA Barcode:
GCAGCCTATACTATAGAATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sgsm2 (97761)
Gene Description:
small G protein signaling modulator 2
Transcript:
RefSeq NM_197943.1 (NON-CURRENT)
Match location:
Position 2525 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093182)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCCTATACTATAGAATTA
Hairpin Sequence:
5'-CCGG-GCAGCCTATACTATAGAATTA-CTCGAG-TAATTCTATAGTATAGGCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCCTATACTATAGAATTACTCGAGTAATTCTATAGTATAGGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCCTATACTATAGAATTACTCGAGTAATTCTATAGTATAGGCTGC-3'

Other clones with same target sequence:

(none)