Construct: shRNA TRCN0000093230

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009307.2-994s1c1
DNA Barcode:
CCCAGCCTTCAATGAGACATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Syt2 (20980)
Gene Description:
synaptotagmin II
Transcript:
RefSeq NM_009307.2 (NON-CURRENT)
Match location:
Position 994 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093230)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGCCTTCAATGAGACATT
Hairpin Sequence:
5'-CCGG-CCCAGCCTTCAATGAGACATT-CTCGAG-AATGTCTCATTGAAGGCTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGCCTTCAATGAGACATTCTCGAGAATGTCTCATTGAAGGCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGCCTTCAATGAGACATTCTCGAGAATGTCTCATTGAAGGCTGGG-3'

Other clones with same target sequence:

(none)