Construct: shRNA TRCN0000093236

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001018086.2-542s1c1
DNA Barcode:
CCTGTGGAAACACAAGGAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm4983 (245297)
Gene Description:
predicted gene 4983
Transcript:
RefSeq NM_001018086.2 (NON-CURRENT)
Match location:
Position 542 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093236)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTGGAAACACAAGGAGTT
Hairpin Sequence:
5'-CCGG-CCTGTGGAAACACAAGGAGTT-CTCGAG-AACTCCTTGTGTTTCCACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTGGAAACACAAGGAGTTCTCGAGAACTCCTTGTGTTTCCACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTGGAAACACAAGGAGTTCTCGAGAACTCCTTGTGTTTCCACAGG-3'

Other clones with same target sequence:

(none)