Construct: shRNA TRCN0000093293

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011193.1-1083s1c1
DNA Barcode:
CGGTGCCTTATCAGAACTACT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pstpip1 (19200)
Gene Description:
proline-serine-threonine phosphatase-interacting protein 1
Transcript:
RefSeq NM_011193.1 (NON-CURRENT)
Match location:
Position 1083 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093293)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTGCCTTATCAGAACTACT
Hairpin Sequence:
5'-CCGG-CGGTGCCTTATCAGAACTACT-CTCGAG-AGTAGTTCTGATAAGGCACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTGCCTTATCAGAACTACTCTCGAGAGTAGTTCTGATAAGGCACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTGCCTTATCAGAACTACTCTCGAGAGTAGTTCTGATAAGGCACCG-3'

Other clones with same target sequence:

(none)