Construct: shRNA TRCN0000093309

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178070.2-2353s1c1
DNA Barcode:
GCTGGGAACTTTGGAACTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vps33b (233405)
Gene Description:
vacuolar protein sorting 33B
Transcript:
RefSeq NM_178070.2 (NON-CURRENT)
Match location:
Position 2353 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093309)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGGAACTTTGGAACTAAT
Hairpin Sequence:
5'-CCGG-GCTGGGAACTTTGGAACTAAT-CTCGAG-ATTAGTTCCAAAGTTCCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGGAACTTTGGAACTAATCTCGAGATTAGTTCCAAAGTTCCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGGAACTTTGGAACTAATCTCGAGATTAGTTCCAAAGTTCCCAGC-3'

Other clones with same target sequence:

(none)