Construct: shRNA TRCN0000093336

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010119.3-1243s1c1
DNA Barcode:
CAACGATATAGCTCGGCTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ehd1 (13660)
Gene Description:
EH-domain containing 1
Transcript:
RefSeq NM_010119.3 (NON-CURRENT)
Match location:
Position 1243 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093336)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACGATATAGCTCGGCTGAT
Hairpin Sequence:
5'-CCGG-CAACGATATAGCTCGGCTGAT-CTCGAG-ATCAGCCGAGCTATATCGTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACGATATAGCTCGGCTGATCTCGAGATCAGCCGAGCTATATCGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACGATATAGCTCGGCTGATCTCGAGATCAGCCGAGCTATATCGTTG-3'

Other clones with same target sequence:

(none)