Construct: shRNA TRCN0000093344

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001013774.1-1813s1c1
DNA Barcode:
GCGTGGCTTCTGGCGATAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kpna7 (381686)
Gene Description:
karyopherin alpha 7 (importin alpha 8)
Transcript:
RefSeq NM_001013774.1 (NON-CURRENT)
Match location:
Position 1813 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093344)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTGGCTTCTGGCGATAGTA
Hairpin Sequence:
5'-CCGG-GCGTGGCTTCTGGCGATAGTA-CTCGAG-TACTATCGCCAGAAGCCACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTGGCTTCTGGCGATAGTACTCGAGTACTATCGCCAGAAGCCACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTGGCTTCTGGCGATAGTACTCGAGTACTATCGCCAGAAGCCACGC-3'

Other clones with same target sequence:

(none)