Construct: shRNA TRCN0000093365

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011504.1-1059s1c1
DNA Barcode:
CGAAGCAAGTAGTCCATCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stxbp3 (20912)
Gene Description:
syntaxin binding protein 3
Transcript:
RefSeq NM_011504.1 (CURRENT)
Match location:
Position 1059 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093365)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAAGCAAGTAGTCCATCTTA
Hairpin Sequence:
5'-CCGG-CGAAGCAAGTAGTCCATCTTA-CTCGAG-TAAGATGGACTACTTGCTTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAAGCAAGTAGTCCATCTTACTCGAGTAAGATGGACTACTTGCTTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAAGCAAGTAGTCCATCTTACTCGAGTAAGATGGACTACTTGCTTCG-3'

Other clones with same target sequence:

  1. TRCN0000317099