Construct: shRNA TRCN0000093366

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011504.1-492s1c1
DNA Barcode:
CGGACGCATTCTATTACTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stxbp3 (20912)
Gene Description:
syntaxin binding protein 3
Transcript:
RefSeq NM_011504.1 (CURRENT)
Match location:
Position 492 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093366)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGACGCATTCTATTACTGTT
Hairpin Sequence:
5'-CCGG-CGGACGCATTCTATTACTGTT-CTCGAG-AACAGTAATAGAATGCGTCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGACGCATTCTATTACTGTTCTCGAGAACAGTAATAGAATGCGTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGACGCATTCTATTACTGTTCTCGAGAACAGTAATAGAATGCGTCCG-3'

Other clones with same target sequence:

(none)