Construct: shRNA TRCN0000093371

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019983.2-1182s1c1
DNA Barcode:
CCTGATCTACATCGTCCTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rabgef1 (56715)
Gene Description:
RAB guanine nucleotide exchange factor (GEF) 1
Transcript:
RefSeq NM_019983.2 (CURRENT)
Match location:
Position 1182 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093371)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGATCTACATCGTCCTGAA
Hairpin Sequence:
5'-CCGG-CCTGATCTACATCGTCCTGAA-CTCGAG-TTCAGGACGATGTAGATCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGATCTACATCGTCCTGAACTCGAGTTCAGGACGATGTAGATCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGATCTACATCGTCCTGAACTCGAGTTCAGGACGATGTAGATCAGG-3'

Other clones with same target sequence:

  1. TRCN0000309663