Construct: shRNA TRCN0000093374

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172718.1-4904s1c1
DNA Barcode:
GCCTCCTATTTATTCCATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sgsm1 (52850)
Gene Description:
small G protein signaling modulator 1
Transcript:
RefSeq NM_172718.1 (NON-CURRENT)
Match location:
Position 4904 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093374)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCCTATTTATTCCATTTA
Hairpin Sequence:
5'-CCGG-GCCTCCTATTTATTCCATTTA-CTCGAG-TAAATGGAATAAATAGGAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCCTATTTATTCCATTTACTCGAGTAAATGGAATAAATAGGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCCTATTTATTCCATTTACTCGAGTAAATGGAATAAATAGGAGGC-3'

Other clones with same target sequence:

(none)