Construct: shRNA TRCN0000093380

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_125851.4-2297s1c1
DNA Barcode:
CGCAGCTTGACATACAGATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eea1 (216238)
Gene Description:
early endosome antigen 1
Transcript:
RefSeq XM_125851.4 (NON-CURRENT)
Match location:
Position 2297 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093380)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCAGCTTGACATACAGATTA
Hairpin Sequence:
5'-CCGG-CGCAGCTTGACATACAGATTA-CTCGAG-TAATCTGTATGTCAAGCTGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCAGCTTGACATACAGATTACTCGAGTAATCTGTATGTCAAGCTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCAGCTTGACATACAGATTACTCGAGTAATCTGTATGTCAAGCTGCG-3'

Other clones with same target sequence:

(none)