Construct: shRNA TRCN0000093397

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024225.2-991s1c1
DNA Barcode:
CGATACTACATGCTCAACATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Snx5 (69178)
Gene Description:
sorting nexin 5
Transcript:
RefSeq NM_024225.2 (NON-CURRENT)
Match location:
Position 991 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093397)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGATACTACATGCTCAACATA
Hairpin Sequence:
5'-CCGG-CGATACTACATGCTCAACATA-CTCGAG-TATGTTGAGCATGTAGTATCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGATACTACATGCTCAACATACTCGAGTATGTTGAGCATGTAGTATCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGATACTACATGCTCAACATACTCGAGTATGTTGAGCATGTAGTATCG-3'

Other clones with same target sequence:

(none)