Construct: shRNA TRCN0000093429

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010026.2-4332s1c1
DNA Barcode:
CCTGAGATATTACCTCATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Asap1 (13196)
Gene Description:
ArfGAP with SH3 domain, ankyrin repeat and PH domain1
Transcript:
RefSeq NM_010026.2 (NON-CURRENT)
Match location:
Position 4332 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093429)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAGATATTACCTCATTAT
Hairpin Sequence:
5'-CCGG-CCTGAGATATTACCTCATTAT-CTCGAG-ATAATGAGGTAATATCTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAGATATTACCTCATTATCTCGAGATAATGAGGTAATATCTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAGATATTACCTCATTATCTCGAGATAATGAGGTAATATCTCAGG-3'

Other clones with same target sequence:

(none)