Construct: shRNA TRCN0000093430

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010026.2-516s1c1
DNA Barcode:
GCACAAGTTCTGGATAAGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Asap1 (13196)
Gene Description:
ArfGAP with SH3 domain, ankyrin repeat and PH domain1
Transcript:
RefSeq NM_010026.2 (NON-CURRENT)
Match location:
Position 516 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093430)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAAGTTCTGGATAAGTTT
Hairpin Sequence:
5'-CCGG-GCACAAGTTCTGGATAAGTTT-CTCGAG-AAACTTATCCAGAACTTGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAAGTTCTGGATAAGTTTCTCGAGAAACTTATCCAGAACTTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAAGTTCTGGATAAGTTTCTCGAGAAACTTATCCAGAACTTGTGC-3'

Other clones with same target sequence:

  1. TRCN0000324061