Construct: shRNA TRCN0000093444

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016807.1-1545s1c1
DNA Barcode:
CGCCTTAACTACAGTAAGCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sdcbp (53378)
Gene Description:
syndecan binding protein
Transcript:
RefSeq NM_016807.1 (NON-CURRENT)
Match location:
Position 1545 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093444)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCTTAACTACAGTAAGCAT
Hairpin Sequence:
5'-CCGG-CGCCTTAACTACAGTAAGCAT-CTCGAG-ATGCTTACTGTAGTTAAGGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCTTAACTACAGTAAGCATCTCGAGATGCTTACTGTAGTTAAGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCTTAACTACAGTAAGCATCTCGAGATGCTTACTGTAGTTAAGGCG-3'

Other clones with same target sequence:

(none)