Construct: shRNA TRCN0000093450

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019535.2-1139s1c1
DNA Barcode:
CCCATCAACTATGTAGAAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sh3gl2 (20404)
Gene Description:
SH3-domain GRB2-like 2
Transcript:
RefSeq NM_019535.2 (CURRENT)
Match location:
Position 1139 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093450)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATCAACTATGTAGAAATT
Hairpin Sequence:
5'-CCGG-CCCATCAACTATGTAGAAATT-CTCGAG-AATTTCTACATAGTTGATGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATCAACTATGTAGAAATTCTCGAGAATTTCTACATAGTTGATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATCAACTATGTAGAAATTCTCGAGAATTTCTACATAGTTGATGGG-3'

Other clones with same target sequence:

(none)