Construct: shRNA TRCN0000093474

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020578.1-3247s1c1
DNA Barcode:
CCTCCCGTTCATTAGCCTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ehd3 (57440)
Gene Description:
EH-domain containing 3
Transcript:
RefSeq NM_020578.1 (NON-CURRENT)
Match location:
Position 3247 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093474)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCCGTTCATTAGCCTAAA
Hairpin Sequence:
5'-CCGG-CCTCCCGTTCATTAGCCTAAA-CTCGAG-TTTAGGCTAATGAACGGGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCCGTTCATTAGCCTAAACTCGAGTTTAGGCTAATGAACGGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCCGTTCATTAGCCTAAACTCGAGTTTAGGCTAATGAACGGGAGG-3'

Other clones with same target sequence:

  1. TRCN0000334901