Construct: shRNA TRCN0000093527

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021482.1-169s1c1
DNA Barcode:
GACAATGAGACTGTGCGCTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Syngr4 (58867)
Gene Description:
synaptogyrin 4
Transcript:
RefSeq NM_021482.1 (NON-CURRENT)
Match location:
Position 169 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093527)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACAATGAGACTGTGCGCTTT
Hairpin Sequence:
5'-CCGG-GACAATGAGACTGTGCGCTTT-CTCGAG-AAAGCGCACAGTCTCATTGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACAATGAGACTGTGCGCTTTCTCGAGAAAGCGCACAGTCTCATTGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACAATGAGACTGTGCGCTTTCTCGAGAAAGCGCACAGTCTCATTGTC-3'

Other clones with same target sequence:

(none)