Construct: shRNA TRCN0000093563

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007461.1-1952s1c1
DNA Barcode:
CCAGAAGGAATATAGCGACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Apba2 (11784)
Gene Description:
amyloid beta (A4) precursor protein-binding, family A, member 2
Transcript:
RefSeq NM_007461.1 (NON-CURRENT)
Match location:
Position 1952 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093563)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAAGGAATATAGCGACAT
Hairpin Sequence:
5'-CCGG-CCAGAAGGAATATAGCGACAT-CTCGAG-ATGTCGCTATATTCCTTCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAAGGAATATAGCGACATCTCGAGATGTCGCTATATTCCTTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAAGGAATATAGCGACATCTCGAGATGTCGCTATATTCCTTCTGG-3'

Other clones with same target sequence:

(none)