Construct: shRNA TRCN0000093570

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139302.1-237s1c1
DNA Barcode:
CGAGTGGAGGAGTTCCTATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sh3glb2 (227700)
Gene Description:
SH3-domain GRB2-like endophilin B2
Transcript:
RefSeq NM_139302.1 (NON-CURRENT)
Match location:
Position 237 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093570)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGTGGAGGAGTTCCTATAT
Hairpin Sequence:
5'-CCGG-CGAGTGGAGGAGTTCCTATAT-CTCGAG-ATATAGGAACTCCTCCACTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGTGGAGGAGTTCCTATATCTCGAGATATAGGAACTCCTCCACTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGTGGAGGAGTTCCTATATCTCGAGATATAGGAACTCCTCCACTCG-3'

Other clones with same target sequence:

  1. TRCN0000324389