Construct: shRNA TRCN0000093609

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013933.2-1498s1c1
DNA Barcode:
GCTAATATCATGGCAGAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vapa (30960)
Gene Description:
vesicle-associated membrane protein, associated protein A
Transcript:
RefSeq NM_013933.2 (NON-CURRENT)
Match location:
Position 1498 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093609)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAATATCATGGCAGAATTT
Hairpin Sequence:
5'-CCGG-GCTAATATCATGGCAGAATTT-CTCGAG-AAATTCTGCCATGATATTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAATATCATGGCAGAATTTCTCGAGAAATTCTGCCATGATATTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAATATCATGGCAGAATTTCTCGAGAAATTCTGCCATGATATTAGC-3'

Other clones with same target sequence:

  1. TRCN0000380637