Construct: shRNA TRCN0000093676

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_134189.2-327s1c1
DNA Barcode:
GCTGAAGGACTGGCATAACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Galnt10 (171212)
Gene Description:
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 10
Transcript:
RefSeq NM_134189.2 (CURRENT)
Match location:
Position 327 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093676)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAAGGACTGGCATAACAA
Hairpin Sequence:
5'-CCGG-GCTGAAGGACTGGCATAACAA-CTCGAG-TTGTTATGCCAGTCCTTCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAAGGACTGGCATAACAACTCGAGTTGTTATGCCAGTCCTTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAAGGACTGGCATAACAACTCGAGTTGTTATGCCAGTCCTTCAGC-3'

Other clones with same target sequence:

(none)