Construct: shRNA TRCN0000093765

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011673.2-1018s1c1
DNA Barcode:
CCATGTATTCAGATGGGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ugcg (22234)
Gene Description:
UDP-glucose ceramide glucosyltransferase
Transcript:
RefSeq NM_011673.2 (NON-CURRENT)
Match location:
Position 1018 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093765)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGTATTCAGATGGGATAT
Hairpin Sequence:
5'-CCGG-CCATGTATTCAGATGGGATAT-CTCGAG-ATATCCCATCTGAATACATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGTATTCAGATGGGATATCTCGAGATATCCCATCTGAATACATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGTATTCAGATGGGATATCTCGAGATATCCCATCTGAATACATGG-3'

Other clones with same target sequence:

  1. TRCN0000316734