Construct: shRNA TRCN0000093773

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011372.1-939s1c1
DNA Barcode:
GCCAAGAAGCACAGAATAGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
St6galnac3 (20447)
Gene Description:
ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 3
Transcript:
RefSeq NM_011372.1 (NON-CURRENT)
Match location:
Position 939 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093773)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAGAAGCACAGAATAGTA
Hairpin Sequence:
5'-CCGG-GCCAAGAAGCACAGAATAGTA-CTCGAG-TACTATTCTGTGCTTCTTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAGAAGCACAGAATAGTACTCGAGTACTATTCTGTGCTTCTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAGAAGCACAGAATAGTACTCGAGTACTATTCTGTGCTTCTTGGC-3'

Other clones with same target sequence:

(none)