Construct: shRNA TRCN0000093786

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173030.1-588s1c1
DNA Barcode:
CCTGTACTTCAGCGAGTGTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Galnt13 (271786)
Gene Description:
UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 13
Transcript:
RefSeq NM_173030.1 (NON-CURRENT)
Match location:
Position 588 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093786)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTACTTCAGCGAGTGTAA
Hairpin Sequence:
5'-CCGG-CCTGTACTTCAGCGAGTGTAA-CTCGAG-TTACACTCGCTGAAGTACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTACTTCAGCGAGTGTAACTCGAGTTACACTCGCTGAAGTACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTACTTCAGCGAGTGTAACTCGAGTTACACTCGCTGAAGTACAGG-3'

Other clones with same target sequence:

(none)