Construct: shRNA TRCN0000093790

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010162.1-1762s1c1
DNA Barcode:
CCTTCTACAATCAGGTCTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ext1 (14042)
Gene Description:
exostoses (multiple) 1
Transcript:
RefSeq NM_010162.1 (NON-CURRENT)
Match location:
Position 1762 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000093790)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCTACAATCAGGTCTATT
Hairpin Sequence:
5'-CCGG-CCTTCTACAATCAGGTCTATT-CTCGAG-AATAGACCTGATTGTAGAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCTACAATCAGGTCTATTCTCGAGAATAGACCTGATTGTAGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCTACAATCAGGTCTATTCTCGAGAATAGACCTGATTGTAGAAGG-3'

Other clones with same target sequence:

  1. TRCN0000349517